Research article

Abstract
Conventional solution-phase method currently employed for the production of Antibody-drug Conjugates (ADC) has certain drawbacks, which makes production inefficient and cost extensive process. In this study, we developed a rigorous cost model for an intensified solid-phase production method utilizing a packed bed resin column to carry out all the steps involved in the production of ADC, at an industrial scale.

The aim of this study was to find a suitable operating region (column length and feed velocity) to minimize the cost of ADC production. Simulation results showed that productivity was maximum when the process was operated at a linear velocity ranging between 120-140 cm/hr and unit cost of ADC production was minimum when column length was 60 cm and operated at 100 cm/hr. We found that production cost is highly dependent on monoclonal antibody (mAb) cost, followed by cytotoxic drug as being the second-highest contributor to the overall production cost.

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Authors: Anirudh Gairola, Ahmad K. Hilaly*, M. Nazmul Karim [Author Information]

*Artie McFerrin Department of Chemical Engineering, Texas A&M University, College Station, Texas 778843-3122 | Corresponding Author: Ahmad K. Hilaly;  E-mail: [email protected]. Phone: +1979-458-0090

Key terms: ADC, cost modeling, simulation, solid-phase production, process intensification
Published In: ADC Review| Journal of Antibody-drug Conjugates

DOI: https://doi.org/10.14229/jadc.2020.02.17.001


How to cite:
Gairola A, Hilaly AK*, Karim MN. Modeling and Optimization of Antibody-drug Conjugate Production Using Process Intensified Solid Phase Method – J. ADC. February 17, 2020. DOI: 10.14229/jadc.2020.02.001.


Last Editorial Review: February 11, 2020

Creative Commons License

Article History:

    • Original Manuscript Received December 2, 2019
    • Review results received January 17, 2020
    • Manuscript accepted for publication January 31, 2020
    • First Published Online February 17, 2020.

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Ahmad K. Hilaly, Ph.D, has more than 22 years of industrial experience in the biochemical industry, and extensive experience in research and development management. His research area includes food, nutraceuticals, feed, fuels, and renewable chemicals. He has held positions of process engineer, R&D manager, and R&D director. Hilaly has 16 issued U.S. patents. His teaching interests include process design, mass transfer, and bioprocess engineering.
Anirudh Gairola is an Engineering graduate student, pursuing a Ph.D. degree focused in Chemical Engineering from Texas A&M University, with knowledge of cGMP, lean six sigma tools and principles, downstream and upstream processes, and strong technical background in the biochemical and allied field. Experienced in process designing using SuperPro designer. Skilled in MATLAB, JMP, Excel, and VMD.
Professor M. Nazmul (Naz) Karim is the Michael O’Connor Chair II and the Head of the Artie McFerrin Department of Chemical Engineering at Texas A & M University (TAMU). He received his BS degree in Chemical Engineering from Bangladesh University of Engineering and Technology, and his MSc and Ph.D. degrees from the University of Manchester, UK. He has been a professor of chemical engineering at Colorado State University for over twenty years. He also held the Whitacre Endowed Chair and served as the Department Chair of Chemical Engineering at Texas Tech University (TTU) for eight years before joining TAMU. Karim passed away on Friday, June 21, 2019 in Delft, The Netherlands, while on a work trip.