Peer Reviewed Article:
Validating Protein Concentration of Antibody-Drug Conjugates

Download the Full Article
Explore product features

RESEARCH BRIEF | AUGUST 31 2022

Validating Protein Concentration of Antibody-Drug Conjugates with the CTechTM SoloVPE® System

Nigel Herbert
Sr. Bioanalytics Applications Specialist - Repligen Corporation

Joshua Das
Scientist I, Analytical Research and Development, Global NBE Analytical R&D - AbbVie

Priya Patel
Scientist I, Analytical Research and Development, Global NBE Analytical R&D - AbbVie

Abstract
Antibody-drug conjugates (ADCs) are complex molecules designed to target and kill tumor cells. ADCs consist of a monoclonal antibody, drug linker, and drug (payload), where the antibody generally targets the protein on the tumor cell. UV-Vis spectroscopy is the simplest and most common method to determine protein concentration of ADCs; however, traditional fixed-pathlength spectrophotometers have issues analyzing these complex molecules. The CTechTM SoloVPE® System variable pathlength method, also known as the Slope Spectroscopy® method, offers a more accurate solution. This publication demonstrates how an assay by the SoloVPE method was validated to determine the protein concentration of an ADC. The method was validated by assessing the precision, accuracy, and linearity of the sample. The SoloVPE passed all acceptance criteria seamlessly, proving the technology to be the superior method for this application.

Keywords:
ADC; antibody–drug conjugate; cytotoxic payload; linker; monoclonal antibody; UV-Vis spectroscopy; protein concentration.

How to cite:
Herbert N, Das J, Patel P. Validating Protein Concentration of Antibody-Drug Conjugates with the CTechTM SoloVPE® System – J. ADC. August 31, 2022 . DOI: https://doi.org/10.14229/jadc.2022.08.31.001.

Article History:
Original Manuscript Received: August 1, 2022
Review results received: August 25, 2022
Last Editorial Review: August 29, 2022
Manuscript accepted for publication: August 29, 2020

Conflict of interest statement
The authors declare no conflict of interest.

This content is only available via PDF.

© 2022 ADC Review | Journal of Antibody-drug Conjugates

Click here to Download the Full Article

Repligen Corporation | Headquarters

41 Seyon Street
Building 1, Suite 100
Waltham, Massachusetts 02453
USA

CTech™ SoloVPE® System



The CTech™ SoloVPE® System leverages the power and flexibility of variable pathlength technology to create a rapid, robust, and repeatable, non-disruptive concentration measurement method for proteins, plasmid DNA, and nucleic acid modalities with results in minutes, not hours.

LEARN MORE