IMGN779 Induces DNA Damage, Cell Cycle Arrest, and Apoptosis in AML Cells

CD33 is broadly expressed on leukemic blasts of patients with acute myeloid leukemia. [1] Although nearly 80% of patients show a high initial response rates to chemotherapy, many patients with acute myeloid leukemia (AML) experience a relapse of the disease due to the outgrowth of persistent leukemic stem cells or LSC. Researchers have found that the differential expression of CD33 on leukemic stem cells compared to normal hematopoietic stem cells (HSC) makes CD33 attractive as a target for treatment of acute myeloid leukemia.[1][2]

Data presented during the 57th American Society of Hematology (ASH) Annual Meeting and Exposition which took place in Orlando, Florida, December 5-8, 2015, shows that IMGN779, a novel, experimental, CD33-targeting antibody-drug conjugate utilizing the humanized antibody Z4681A conjugated to DGN462, a novel DNA-alkylating agent consisting of an indolino-benzodiazepine dimer containing a mono-imine moiety, through a cleavable disulfide linker, sulfo-SPDB, is expected to make a meaningful potential in the treatment of patients with acute myeloid leukemia (AML). The novel ADC is being developed by ImmunoGen, Inc., Waltham, MA. [3]

Photo: Exhibition hall. 57th American Society of Hematology (ASH) Annual Meeting and Exposition which took place in Orlando, Florida, December 5-8, 2015.

While most cases of acute myeloid leukemia, also known as acute myelocytic leukemia, acute myelogenous leukemia, acute granulocytic leukemia, and acute non-lymphocytic leukemia, develop from cells that turn into white blood cells (other than lymphocytes), some cases develop in other types of blood-forming cells.

ADC Bio
Lonza
MabPlex
 

Staring in the bone marrow, AML, in most cases, quickly moves into the blood and,  sometimes, spread to other parts of the body including the lymph nodes, liver, spleen, central nervous system (including brain and spinal cord), and testicles. [4]

Key statistics
Acute myeloid leukemia, which is slightly more common among men than among women, is generally a disease of older people and is uncommon before the age of 45. The average age of a patient with AML is about 67 years. In 2015 in the United States, according to the American Cancer Society, about 20,830 new cases of acute myeloid leukemia have been diagnosed. Most of them in adults. Furthermore, about 10,460 died from AML. [4]

In people younger than 60 years of age, treatment of AML is fairly standard, involving cycles of intensive chemotherapy including cytarabine (cytosine arabinoside or ara-C) and the anthracycline drugs (such as daunorubicin (daunomycin), idarubicin, and mitoxantrone). This may, sometimes, go along with a stem cell transplant. However, in patients older than 60 years of age, this may be different. If older patients are healthy enough to be treated in the same way, the chemotherapy may be less intense. However, people who are much older or who are in poor health may not be able to tolerate this intense treatment, creating a large unmet need. [4] Novel, targeted therapies may be able address this need.

Meaningful difference
Study results presented this year, show how researchers investigated the mechanism of action of DGN462 and IMGN779, using AML cell lines and primary patient AML samples to evaluate DNA binding, DNA alkylation versus cross-linking, cell cycle effects and DNA damage signaling, apoptosis, and cell death.

The results suggest that cell killing by DGN462 and IMGN779 are highly potent against AML cell lines in vitro and in primary patient AML samples, acting mechanistically via induction of DNA damage, which is accompanied by cell cycle arrest, leading to apoptosis.

In this study researchers induced phosphorylated H2AX (a marker of DNA damage) and cleaved Caspase-3 (a markers of apoptosis), in a time dependent manner following exposure to either DGN462 or IMGN779.

Based on the results, IMGN779 is advancing to clinical testing for the treatment of relapsed and/or refractory CD33+ acute myeloid leukemia. In new pharmacodynamic studies, utilizing phosphorylated H2AX as a biomarker, researchers expect to verify the mechanism of DGN462, a novel DNA alkylating payload which covalently binds to cellular DNA, without cross-linking.

“The IMGN779 data being reported relate to its novel mechanism of action, which we believe could help patients with acute myeloid leukemia, and we expect to start clinical testing of this experimental therapy in early 2016,” noted Charles Morris, MBChB, MRCP, ImmunoGen’s Executive Vice President and Chief Development Officer.

Preclinical data was previously presented during the 2014 annual meeting of the American Society of Hematology. [5][6]

“We believe the unique profile of IMGN779 will enable [us to] achieve a desired activity with a better tolerability profile than other agents currently used to treat this disease,” Morris concluded.