Research article
Abstract
Conventional solution-phase method currently employed for the production of Antibody-drug Conjugates (ADC) has certain drawbacks, which makes production inefficient and cost extensive process. In this study, we developed a rigorous cost model for an intensified solid-phase production method utilizing a packed bed resin column to carry out all the steps involved in the production of ADC, at an industrial scale.
The aim of this study was to find a suitable operating region (column length and feed velocity) to minimize the cost of ADC production. Simulation results showed that productivity was maximum when the process was operated at a linear velocity ranging between 120-140 cm/hr and unit cost of ADC production was minimum when column length was 60 cm and operated at 100 cm/hr. We found that production cost is highly dependent on monoclonal antibody (mAb) cost, followed by cytotoxic drug as being the second-highest contributor to the overall production cost.
Authors: Anirudh Gairola, Ahmad K. Hilaly*, M. Nazmul Karim [Author Information]
*Artie McFerrin Department of Chemical Engineering, Texas A&M University, College Station, Texas 778843-3122 | Corresponding Author: Ahmad K. Hilaly; E-mail: ahmad.hilaly@tamu.edu. Phone: +1979-458-0090
Key terms: ADC, cost modeling, simulation, solid-phase production, process intensification
Published In: ADC Review| Journal of Antibody-drug Conjugates
DOI: https://doi.org/10.14229/jadc.2020.02.17.001
How to cite:
Gairola A, Hilaly AK*, Karim MN. Modeling and Optimization of Antibody-drug Conjugate Production Using Process Intensified Solid Phase Method – J. ADC. February 17, 2020. DOI: 10.14229/jadc.2020.02.001.
Last Editorial Review: February 11, 2020
Article History:
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- Original Manuscript Received December 2, 2019
- Review results received January 17, 2020
- Manuscript accepted for publication January 31, 2020
- First Published Online February 17, 2020.
